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Application notes
Hitachi Technical Report FL No. 170002 – Description of Fluorescence Fingerprint Analytical Method

Fluorescence Phenomenon and 3-D Fluorescence Spectra (Fluorescence Fingerprint)


-     A sample is irradiated with light at a variety of wavelengths, and the fluorescence emitted from the compound is observed.


-     The excitation wavelength, fluorescence wavelength, and fluorescence intensity vary with the type and concentration of the compound.


-     The fluorescence properties of the sample (fluorescence fingerprint) can be acquired using the 3-D fluorescence spectrum mode of the fluorophotometer.



With a fluorophotometer after fixing the excitation wavelength and scanning for emission wavelengths, the operation of scanning for fluorescence wavelengths at the next excitation wavelength is repeated to construct 3-D fluorescence spectra.
 

 

Automatic filtering attachment
 



Hitachi F-7100 Fluorescence Spectrophotometer

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