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Application notes
Hitachi Technical Report FL No. 170002 – Description of Fluorescence Fingerprint Analytical Method
Fluorescence Phenomenon and 3-D Fluorescence Spectra (Fluorescence Fingerprint)
- A sample is irradiated with light at a variety of wavelengths, and the fluorescence emitted from the compound is observed.
- The excitation wavelength, fluorescence wavelength, and fluorescence intensity vary with the type and concentration of the compound.
- The fluorescence properties of the sample (fluorescence
fingerprint) can be acquired using the 3-D fluorescence spectrum mode of the
fluorophotometer.
With a fluorophotometer after fixing the excitation
wavelength and scanning for emission wavelengths, the operation of scanning for
fluorescence wavelengths at the next excitation wavelength is repeated to
construct 3-D fluorescence spectra.
Automatic filtering attachment
Hitachi F-7100 Fluorescence Spectrophotometer