ANALYTICAL
CONDITIONS
Instrument : UH5300
Scan speed : 400 nm/min
Slit : 1 nm
Measurement wavelength range : 300 – 800 nm
6-cell mode : Automatic
Intelligent start : ON
Sample : 0.1 mM Bromothymol Blue (BTB)
Solvent : 0.0667 mol/L potassium dihydrogen phosphate (KH2PO4) / 0.0667 mol/L disodium hydrogen phosphate (Na2HPO4)
pH measurement range : About pH 5.9 – pH
7.1
Figure 1 Comparison of Single Cell and
Automatic Analyses
Figure 2 Absorption Spectrum of BTB
Figure 3 Structural Formula of BTB
Figure 4 Appearance of Samples
Instrument
Hitachi UV-Visible Spectrophotometers | UH5300 Double-Beam with Wireless Control Capability
Appearance of 6-cell Turret
Product Info: Hitachi UH5300 UV-VIS Spectrophotometer (Link to https://hk.techcomp.com.hk/EN/product/content/?MenuID=286&ContentID=3269&CID=301) (same window)