Confirmation of Mass Information for Chlorhexidine
Chromaster 5610 MS detector is a new mass detector, designed for LC users, and it is different from conventional mass spectrometers. With this detector and a syringe pump, a sample solution is introduced directly to 5610 MS Detector and the mass information can be obtained. This time, the analysis example of chlorhexidine, a main component of antibacterial disinfectants, is introduced here. A protonated molecular ion could be confirmed by simple operation.
5610 MS Detector
Infusion measurement of Chlorhexidine
n Analytical Conditions
Ionization method |
ESI |
Ionization mode |
Positive |
Ionization voltage |
2300 V |
Measurement mode |
Scan |
Gas flow rate |
0.7 L/min |
IS/AIF temperature |
100 ?C / 120 ?C |
Pump flow rate |
2 μL/min |
Table 1 MS Detector Setting Conditions
n Sample Preparation
Chrolhexidine: |
C22H30Cl2N10 |
Concentration: |
1 mg/mL |
Solvent: |
CH3OH |
Fig. 1 Structure Formula of Chrorhexidine
MS Spectrum
Fig. 3 Mass Spectrum of Chlorhexidine
Measurement Method
By connecting the syringe pump and MS Detector, the sample solution is directly delivered.
A protonated molecular ion, [M+H]+, and an isotope peak were observed. MS information can be obtained by a few milliliters sample volume, by saving the effort of starting up LC.
Main system configuration: Chromaster 5610 MS Detector, Syringe pump, Data processing unit
Product Info: Hitachi 5610 MSD
For more information on Hitachi MSD, please visit Hitachi High-Tech Science website
https://www.hitachi-hightech.com/global/product_detail/?pn=ana-chromaster-5610