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Application notes
The rapid measurement of the wavelength scan for multi samples by UH5300 - Absorption Spectrum Measu

ANALYTICAL CONDITIONS

Instrument : UH5300

Scan speed : 400 nm/min

Slit : 1 nm

Measurement wavelength range : 300 – 800 nm

6-cell mode : Automatic

Intelligent start : ON

Sample : 0.1 mM Bromothymol Blue (BTB)

Solvent : 0.0667 mol/L potassium dihydrogen phosphate (KH2PO4) / 0.0667 mol/L disodium hydrogen phosphate (Na2HPO4)

pH measurement range : About pH 5.9 – pH 7.1


Figure 1 Comparison of Single Cell and Automatic Analyses



Figure 2 Absorption Spectrum of BTB



Figure 3 Structural Formula of BTB


Figure 4 Appearance of Samples


Instrument


Hitachi UV-Visible Spectrophotometers | UH5300 Double-Beam with Wireless Control Capability


Appearance of 6-cell Turret

Product Info: Hitachi UH5300 UV-VIS Spectrophotometer (Link to https://hk.techcomp.com.hk/EN/product/content/?MenuID=286&ContentID=3269&CID=301)     (same window)

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