×
×
×
×
×

Home· · ·Techcomp college· · ·Application notes

Application notes
Analysis of DNA by by U-5100 UV/VIS Spectrophotometer

Analysis of DNA by U-5100 UV/VIS Spectrophotometer

DNA shows absorption in a specific wavelength region. Therefore the absorbance measurement allows the concentration determination and purity confirmation. The absorbance of 1 at 260 nm generally indicates 50 pg of double-stranded DNA (dsDNA), 33 pg of single-stranded DNA (ssDNA), or 40 pg Of RNA and thus, a nucleic acid concentration can be calculated based on the absorbance at 260 nm. Furthermore, as the absorption maximum for proteins occurs at 280 nm, by calculating with respect to the nucleic acid absorbance at 260 nm and comparing with the expected value the purity can be confirmed from the protein contamination ratio. This time Lambda DNA was analyzed by using a commercially available disposable micro cell.
 

Absorbance Spectrum Measurement for Lambda DNA
When a calibration curve of Lambda DNA was prepared for the range of 2 – 60 ng/μL, a good correlation result (R2 = 0.999) was obtained.
The absorbance 
ratio (A260/280) for Lambda DNA 30ng/μL was 1.96.  For DNA, 1.8 or higher ratio is considered high purity and thus, it was indicated that the purity of this sample was high.
By selecting the low speed for the response under the analytical conditions, the noise can be reduced.

 
Analytical Conditions

 Instrument

 U-5100 UV/VIS Spectrophotometer

 Measurement wavelength range

 230-330 nm

 Scan speed

 200 nm/min

 Slit

 5 nm

 Sampling interval

 1 nm

 Response

 Low speed


 
Accessory

Single cell holder (p/n 3J2-0110)

 
Sample
Sample: Lambda DNA
Solvent: TE buffer (10nM Tris HCl, 1nM EDTA)
 
Calibration Curve of Lambda DNA

  
Absorption Spectrum of Lambda DNA

 

Product information:





Online PDF reading

Online PDF reading

top
Micro-blog Sina Facebook

Contact UsMore

Hong Kong
  • 2606, 26/F., Tower 1, Ever Gain Plaza, 88 Container Port Road, Kwai Chung, N.T., Hong Kong
  • +852-27519488 / WhatsApp/WeChat HK: +852-8491 7250
  • techcomp@techcomp.com.hk
Singapore
  • 2 International Business Park, #09-06 Tower 1 The Strategy, Singapore 609930
  • +65-62678921
  • techcomp@techcomp.com.sg

powered by QianYe

All Right Reserved 1988-2025©TECHCOMP GROUP
× Sweep The Concern Us