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Application notes
Confirmation of Mass Information by Online Solvent Extraction of Components Separated by TLC

In order to confirm the mass information of the components separated by thin layer chromatography (TLC), a time-consuming manual procedure consisting of (1) removal of the spot, (2) extraction, (3) analysis by mass analyzer, is often performed.  By using the CAMAG TLC-MS Interface 2 (CAMAG) which automates the online solvent extraction for the above procedure, the extraction and introduction of TLC spot to the mass analyzer can be performed easily.


In this study, analysis of various compounds was performed by using CAMAG in combination with the 5610 MS Detector.  Target spots were directly extracted from TLC plates and analyzed, and the results are presented in this note.




Figure 1 - Example of TLC-MS System Configuration





Figure 2 – Mass Spectra of Each Spot Extracted from TLC plate

 

Please download the technical note for more details.

 

For more information on Hitachi HPLC-MSD, please visit Hitachi High-Tech Science website

https://www.hitachi-hightech.com/global/products/science/ai/

 

MSD 5610 Product Information

http://hk.techcomp.com.hk/EN/product/content/?MenuID=23&ContentID=1859&CID=218


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