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Application notes
Confirmation of Mass Information by Online Solvent Extraction of Components Separated by TLC
In order to confirm the mass information of the components
separated by thin layer chromatography (TLC), a time-consuming manual procedure
consisting of (1) removal of the spot, (2) extraction, (3) analysis by mass
analyzer, is often performed. By using
the CAMAG TLC-MS Interface 2 (CAMAG) which automates the online solvent
extraction for the above procedure, the extraction and introduction of TLC spot
to the mass analyzer can be performed easily.
In this study, analysis of various
compounds was performed by using CAMAG in combination with the 5610 MS
Detector. Target spots were directly
extracted from TLC plates and analyzed, and the results are presented in this
note.
Figure 1 - Example of TLC-MS System Configuration
Figure 2 – Mass Spectra of Each Spot Extracted from TLC plate
Please download the technical note for more details.
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MSD 5610 Product Information
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