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Are you choosing the right sterilizing program?
Tomy
Autoclave
1. Liquid
sterilizing program
Taking
the common LB medium as an example, after pouring the medium with proper pH
into the bottle, then choose the liquid sterilizing program,which usually
takes longer time.
That
is because the exhausting and cooling speed of liquid sterilizing course is
zero. Why?
If any
of you have encountered such a situation——You are urgent to use
medium, but the temperature of the freshly sterilized medium is too high to use.
So you just put the medium under cold
water to cool down. At this moment, a "pop" sound was heard, and the
bottle of medium was scrapped——it cannot be used anymore. This
phenomenon is called sudden boiling. We
need to avoid a sudden drop in temperature when sterilizing liquids.
2. Sterilizing program
When
the sterilized items are mainly solid, such as blue cap bottles, conical
bottles, petri dishes, cell spreaders and pipette tips, we can choose the normal
sterilizing program of which the exhausting and cooling speed will be much
faster than liquid sterilization.
3. Sterilizing-warming course
This
feature is very friendly for those who do not have time to take bottles out in
time after sterilization. The medium with agar can be kept warm in a TOMY
autoclave after sterilization is complete. (Press and hold FUNCTION to manually
adjust the holding time as needed)
4. Heating-warming program (SX series)
What
shall we do if the oven is full? This function of the TOMY autoclave may be
little known: dissolving medium and keeping it warm. In this course, the
autoclave will heat and dissolve the medium first and then enter the heat
preservation program. (Press and hold FUNCTION to manually adjust the holding
time as needed)
Correctly selecting the sterilization courses helps improve efficiency of the experiment and maintenance of the autoclave.
For more product info about Tomy Autoclave, please visit http://hk.techcomp.com.hk/EN/product/?MenuID=24&CID=210
Source: TechcompChina