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Application notes
3D Fluorescent Spectra (Fluorescence Fingerprint) Measurement of Milk Powder

In 2008, there was a problem of melamine being mixed with milk powder to falsely claim the protein content. In order to confirm the melamine content in milk powder, instruments such as HPLC, CMC and LCMS are used. However, the preparation is cumbersome and it takes a long time to analyze.

This time, the 3D fluorescence spectra (fluorescence fingerprint) of milk powder, melamine, and the mixture were obtained from their powder forms without any preparation.

As a result of the analysis, four characteristic fluorescence peaks of (i) to (iv) were confirmed from the milk powder while fluorescence peaks of (v) and (iv) were confirmed from the melamine powder. Three types of milk powder were mixed with melamine powder at the ration of 10:1 and by using the six wavelengths, multivariate statistics were preformed to analyze the main component. The result of the main component analysis indicated that the main component score shifts to the direction for melamine when milk powder is mixed with melamine powder.



 




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